What is the difference between nucleotide and nucleoside?


Nucleotides and nucleosides are both essential components of nucleic acids, such as DNA and RNA, but they differ in their composition and function. Here are five key differences between nucleotides and nucleosides:

Composition:

Nucleotide: A nucleotide consists of three main components – a nitrogenous base, a pentose sugar (either ribose or deoxyribose), and a phosphate group. The presence of the phosphate group distinguishes nucleotides from nucleosides.
Nucleoside: A nucleoside lacks the phosphate group found in nucleotides. It is composed of a nitrogenous base and a pentose sugar but does not contain the phosphate moiety.
Phosphate Group:

Nucleotide: Nucleotides contain one, two, or three phosphate groups linked to the 5′ carbon of the sugar molecule. The number of phosphate groups distinguishes between mono-, di-, and triphosphate nucleotides.
Nucleoside: Nucleosides do not have a phosphate group. The absence of the phosphate group is a fundamental distinction from nucleotides.
Function:

Nucleotide: Nucleotides serve as the building blocks of nucleic acids (DNA and RNA). They are involved in the storage and transfer of genetic information.
Nucleoside: Nucleosides are involved in the synthesis of nucleotides. They can be phosphorylated by kinases to form nucleotides. Additionally, nucleosides play roles in cellular energy transfer (e.g., adenosine in ATP).
Energy Storage:

Nucleotide: Nucleotides, especially triphosphate nucleotides like ATP (adenosine triphosphate), are crucial for storing and transferring energy within cells.
Nucleoside: Nucleosides, lacking the phosphate groups, are not directly involved in energy storage or transfer.
Examples:

Nucleotide: Examples of nucleotides include ATP (adenosine triphosphate), GTP (guanosine triphosphate), CTP (cytidine triphosphate), UTP (uridine triphosphate), and dATP (deoxyadenosine triphosphate).
Nucleoside: Examples of nucleosides include adenosine, guanosine, cytidine, uridine, and thymidine. These are the basic units that can be phosphorylated to form nucleotides.
In summary, while both nucleotides and nucleosides are essential components of nucleic acids, nucleotides include a phosphate group and are involved in genetic information transfer and energy storage, whereas nucleosides lack the phosphate group and are intermediates in nucleotide synthesis.